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The pooled data from seven low- and middle-income Asian countries including China showed that MSM were 18.7 times more likely to have HIV infection than other reproductive-age men [2].The risk factors associated with HIV infection among MSM include both biological and behavioral factors such as unprotected anal intercourse (UAI), substance abuse, and co-infection with other sexually transmitted infections (STIs) like syphilis and herpes simplex virus type 2 (HSV-2) [1, 3–6].The median age of all participants was 26 years (IQR: 23–33 years), and the majority were single (76%), of Han ethnics (93%) and had lived in the study cities for over 6 months.

26.3%), have unprotected anal intercourse (77.9% vs. 6.2%) in the past 6 months (each P Men who have sex with men (MSM) are at a high risk of HIV infection around the world [1].

HSV-2 infection was determined using ELISA (Herpe Select-2®, Focus Diagnostics, Cypress, CA, USA).

Student t-tests were used to calculate means ± standard deviations (SD) for continuous variables and Pearson’s Chi-square test or Fisher’s exact test were used for comparing proportions (%) for categorical variables.

We conducted a cross-sectional survey among MSM in six Chinese cities (Shenyang, Ji’nan, Changsha, Zhengzhou, Nanjing, and Kunming) from 2012 to 2013.

Data on socio-demographics and sexual behaviors were collected.

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